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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
24/08/2017 |
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Data da última atualização: |
23/09/2019 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
BATISTA, R. I. T. P.; CAMARGO, L. S. de A.; SOUZA FABJAN, J. M. G.; PRATES, J. F.; TREVIZAN, J. T.; BRANDÃO, F. Z.; FONSECA, J. F. da. |
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Afiliação: |
Ribrio Ivan Tavares Pereira Batista; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; Joanna Maria Gonçalves de Souza Fabjan, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; Jader Forquim Prates; Juliane Teramachi Trevizan; Felipe Zandonadi Brandao; JEFERSON FERREIRA DA FONSECA, CNPC. |
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Título: |
Goat incubator: the doe as a life incubator of bovine oocytes - first step. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Animal Reprodroduction, v. 14, n. 3, p. 738, Jul./Sept. 2017. |
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Idioma: |
Inglês |
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Notas: |
Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts. |
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Conteúdo: |
Despite significant improvements in the in vitro production of cattle embryos, the suboptimal in vitro culture environment still limits the embryo quality and production. Techniques that associate the advantages of in vivo and in vitro systems, such as intrafollicular transfer of immature oocytes, have been proposed mainly to increase the embryo quality. In this context, we tried to use a goat as live incubator and associated nonsurgical embryo transfer techniques in small ruminants to perform ex situ (in vivo) maturation of bovine oocytes. For this, immature bovine cumulus-oocyte complexes (COCs) of grade 1 and 2 were randomly distributed into two groups for in vitro (IVM; n = 38) and ex situ (ESM; n = 40) maturation. The IVM was performed for a period of 24 h in TCM-199 medium (Gibco Life Technologies, Inc., Grand Island, NY, USA) supplemented with 20 mg/mL of FSH (Pluset, Calier, Barcelona, Spain), 0.36 mM sodium pyruvate (Sigma Chemical, St. Louis, MO, USA), 10 mM sodium bicarbonate (Sigma Chemical, St. Louis, MO, USA) and 50 mg/mL streptomycin/penicillin (Sigma Chemical, St. Louis, MO, USA) at 38.8 ºC in an atmosphere of 5% CO2 in air with maximum humidity. For ESM, a pre-synchronized nulliparous goat (12 months old) received 40 immature COCs in the uterine horn apice by transcervical route (Fonseca et al., 2014 Arq. Bras. Med.vet. Zootec) and 24 h after the procedure the structures were retrieved by the uterine flushing (Fonseca et al., 2013 Small Rumin Res). For analysis of the nuclear maturation rate and lipid quantification, the oocytes were denuded (0.1% hyaluronidase), fixed (4% paraformaldehyde) and stained with 10 ?g/mL Hoechst 33342 and 10 ?g/mL Nile Red (Molecular Probes, Inc., Eugene, OR, USA) dissolved in physiological saline (0.9% NaCl) with 1mg/mL polyvinylpyrrolidone. Oocytes displaying metaphase II plate were considered matured. The lipid amount was inferred by measuring the fluorescence intensity using the ImageJ program and fluorescence intensity were compared by Student's t-test. Forty-seven percent of the structures were recovered after uterine flushing (19/40). The nuclear maturation rate was 94.5% (18/19) and 81.6% (31/38) for ESM and IVM groups, respectively. In vitro-matured oocytes contained more lipid droplets, expressed as a higher (p < 0.05) amount of emitted fluorescence light (858 ± 73 arbitrary fluorescence units) than ex situ-matured oocytes (550 ± 64 arbitrary fluorescence units). This is the first report associating nonsurgical embryo transfer techniques with goat as live incubator for maturation of bovine oocytes. We conclude that transcervical transfer of bovine oocytes to uterine goat may be an alternative to in vitro maturation aiming the reduction of lipids without compromising nuclear maturation. Further studies are required to improve the oocyte recovery rate. MenosDespite significant improvements in the in vitro production of cattle embryos, the suboptimal in vitro culture environment still limits the embryo quality and production. Techniques that associate the advantages of in vivo and in vitro systems, such as intrafollicular transfer of immature oocytes, have been proposed mainly to increase the embryo quality. In this context, we tried to use a goat as live incubator and associated nonsurgical embryo transfer techniques in small ruminants to perform ex situ (in vivo) maturation of bovine oocytes. For this, immature bovine cumulus-oocyte complexes (COCs) of grade 1 and 2 were randomly distributed into two groups for in vitro (IVM; n = 38) and ex situ (ESM; n = 40) maturation. The IVM was performed for a period of 24 h in TCM-199 medium (Gibco Life Technologies, Inc., Grand Island, NY, USA) supplemented with 20 mg/mL of FSH (Pluset, Calier, Barcelona, Spain), 0.36 mM sodium pyruvate (Sigma Chemical, St. Louis, MO, USA), 10 mM sodium bicarbonate (Sigma Chemical, St. Louis, MO, USA) and 50 mg/mL streptomycin/penicillin (Sigma Chemical, St. Louis, MO, USA) at 38.8 ºC in an atmosphere of 5% CO2 in air with maximum humidity. For ESM, a pre-synchronized nulliparous goat (12 months old) received 40 immature COCs in the uterine horn apice by transcervical route (Fonseca et al., 2014 Arq. Bras. Med.vet. Zootec) and 24 h after the procedure the structures were retrieved by the uterine flushing (Fonseca et al., 2013 Small Rumin Res). For analy... Mostrar Tudo |
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Palavras-Chave: |
Embryo Technology; Maturation; Non-surgical collection; Tecnologia do embrião. |
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Thesagro: |
Cultura In Vitro; Maturação artificial; Ovelha; Ovino; Reprodução animal. |
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Thesaurus Nal: |
Ewes; In vitro culture; In vitro fertilization; Ova; Reproduction; Sheep. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/163010/1/cnpc-2017-Goat.pdf
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Marc: |
LEADER 04078nam a2200373 a 4500
001 2074430
005 2019-09-23
008 2017 bl uuuu u00u1 u #d
100 1 $aBATISTA, R. I. T. P.
245 $aGoat incubator$bthe doe as a life incubator of bovine oocytes - first step.$h[electronic resource]
260 $aAnimal Reprodroduction, v. 14, n. 3, p. 738, Jul./Sept. 2017.$c2017
500 $aProceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts.
520 $aDespite significant improvements in the in vitro production of cattle embryos, the suboptimal in vitro culture environment still limits the embryo quality and production. Techniques that associate the advantages of in vivo and in vitro systems, such as intrafollicular transfer of immature oocytes, have been proposed mainly to increase the embryo quality. In this context, we tried to use a goat as live incubator and associated nonsurgical embryo transfer techniques in small ruminants to perform ex situ (in vivo) maturation of bovine oocytes. For this, immature bovine cumulus-oocyte complexes (COCs) of grade 1 and 2 were randomly distributed into two groups for in vitro (IVM; n = 38) and ex situ (ESM; n = 40) maturation. The IVM was performed for a period of 24 h in TCM-199 medium (Gibco Life Technologies, Inc., Grand Island, NY, USA) supplemented with 20 mg/mL of FSH (Pluset, Calier, Barcelona, Spain), 0.36 mM sodium pyruvate (Sigma Chemical, St. Louis, MO, USA), 10 mM sodium bicarbonate (Sigma Chemical, St. Louis, MO, USA) and 50 mg/mL streptomycin/penicillin (Sigma Chemical, St. Louis, MO, USA) at 38.8 ºC in an atmosphere of 5% CO2 in air with maximum humidity. For ESM, a pre-synchronized nulliparous goat (12 months old) received 40 immature COCs in the uterine horn apice by transcervical route (Fonseca et al., 2014 Arq. Bras. Med.vet. Zootec) and 24 h after the procedure the structures were retrieved by the uterine flushing (Fonseca et al., 2013 Small Rumin Res). For analysis of the nuclear maturation rate and lipid quantification, the oocytes were denuded (0.1% hyaluronidase), fixed (4% paraformaldehyde) and stained with 10 ?g/mL Hoechst 33342 and 10 ?g/mL Nile Red (Molecular Probes, Inc., Eugene, OR, USA) dissolved in physiological saline (0.9% NaCl) with 1mg/mL polyvinylpyrrolidone. Oocytes displaying metaphase II plate were considered matured. The lipid amount was inferred by measuring the fluorescence intensity using the ImageJ program and fluorescence intensity were compared by Student's t-test. Forty-seven percent of the structures were recovered after uterine flushing (19/40). The nuclear maturation rate was 94.5% (18/19) and 81.6% (31/38) for ESM and IVM groups, respectively. In vitro-matured oocytes contained more lipid droplets, expressed as a higher (p < 0.05) amount of emitted fluorescence light (858 ± 73 arbitrary fluorescence units) than ex situ-matured oocytes (550 ± 64 arbitrary fluorescence units). This is the first report associating nonsurgical embryo transfer techniques with goat as live incubator for maturation of bovine oocytes. We conclude that transcervical transfer of bovine oocytes to uterine goat may be an alternative to in vitro maturation aiming the reduction of lipids without compromising nuclear maturation. Further studies are required to improve the oocyte recovery rate.
650 $aEwes
650 $aIn vitro culture
650 $aIn vitro fertilization
650 $aOva
650 $aReproduction
650 $aSheep
650 $aCultura In Vitro
650 $aMaturação artificial
650 $aOvelha
650 $aOvino
650 $aReprodução animal
653 $aEmbryo Technology
653 $aMaturation
653 $aNon-surgical collection
653 $aTecnologia do embrião
700 1 $aCAMARGO, L. S. de A.
700 1 $aSOUZA FABJAN, J. M. G.
700 1 $aPRATES, J. F.
700 1 $aTREVIZAN, J. T.
700 1 $aBRANDÃO, F. Z.
700 1 $aFONSECA, J. F. da
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Clima Temperado. |
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Data corrente: |
16/02/2008 |
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Data da última atualização: |
30/11/2023 |
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Tipo da produção científica: |
Documentos |
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Autoria: |
RIBEIRO, M. E. R.; STUMPF JUNIOR, W.; GOMES, J. F.; BITENCOURT, D.; BARCELOS, P.; BARBOSA, R. S.; ZONTA, M.; AQUINO, S. L. G.; CANDIA, P. R.; DALBANN, P. R. |
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Afiliação: |
MARIA EDI ROCHA RIBEIRO, CPACT; WALDYR STUMPF JUNIOR, CPACT; JORGE FAINE GOMES, CPACT; DARCY BITENCOURT, CPACT; PATRÍCIA BARCELOS; ROSÂNGELA SILVEIRA BARBOSA, Doutouranda do PPGZ/UFPel; MIRIAM ZONTA; SERGIO LUIS GONCALVES AQUINO, CPACT; PAULO ROBERTO CANDIA, CPACT; PEDRO RENATO DALBANN, CPACT. |
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Título: |
Sistema de coleta e envio de amostras de leite para determinação dos componentes do leite, contagem de células somáticas e contagem bacteriana total. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
Pelotas: Embrapa Clima Temperado, 2007. |
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Páginas: |
22 p. |
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Série: |
(Embrapa Clima Temperado. Documentos, 206). |
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ISSN: |
1806-9193 |
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Idioma: |
Português |
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Conteúdo: |
Esta publicação visa orientar aos produtores e técnicos do setor sobre a rotina de coleta e encaminhamento de amostras de leite do estabelecimento rural até Lableite, de forma a estruturar um processo com qualidade. |
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Palavras-Chave: |
Amostra; Composição. |
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Thesagro: |
Análise; Leite; Qualidade. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/33944/1/documento-206.pdf
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Marc: |
LEADER 01125nam a2200313 a 4500
001 1745859
005 2023-11-30
008 2007 bl uuuu u0uu1 u #d
022 $a1806-9193
100 1 $aRIBEIRO, M. E. R.
245 $aSistema de coleta e envio de amostras de leite para determinação dos componentes do leite, contagem de células somáticas e contagem bacteriana total.
260 $aPelotas: Embrapa Clima Temperado$c2007
300 $a22 p.
490 $a(Embrapa Clima Temperado. Documentos, 206).
520 $aEsta publicação visa orientar aos produtores e técnicos do setor sobre a rotina de coleta e encaminhamento de amostras de leite do estabelecimento rural até Lableite, de forma a estruturar um processo com qualidade.
650 $aAnálise
650 $aLeite
650 $aQualidade
653 $aAmostra
653 $aComposição
700 1 $aSTUMPF JUNIOR, W.
700 1 $aGOMES, J. F.
700 1 $aBITENCOURT, D.
700 1 $aBARCELOS, P.
700 1 $aBARBOSA, R. S.
700 1 $aZONTA, M.
700 1 $aAQUINO, S. L. G.
700 1 $aCANDIA, P. R.
700 1 $aDALBANN, P. R.
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Embrapa Clima Temperado (CPACT) |
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